Citation: Met FANG, Li SHENG, Hut Wan HAN, Xiao YU, Rut ZHAO, Guo Quan LIU. A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis[J]. Chinese Chemical Letters, ;1999, 10(3): 235-238. shu

A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis

Laboratory of Analytical Chemistry in Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080

Received Date: 6 October 1998

Fund Project: The authors acknowledge the supports of the National Natural Seience Foundition of China and "863" Project of High Technique.

A novel capillary electrophoresis method coupled with on-line microdialysis using an attachable electrode has been developed to study the interaction of macromolecule with small molecule.The binding constants of bovine serum album (BSA) with D.L-tryptophan (Trp), sulfamethoxazole (SMZ) with trypsin and chymotrypsin were determined.These values are 2.3×10⁴L/mol for BSA-L-Trp.1.77×10³ L/mol for BSA-D-TrP in PH 7.4, 50 mmol/ L phosphate; 1.4×10⁴ L/mol for SMZ-trypsin and 6.0×10³ L/mol for SMZ-chymotrypsin in PH 6.5, 25 mmol/L Tris buffer.The proposed method has merits of speed.low sample consumption and readily available to be performed in desired physiological conditions.
- Interaction of macromolecule with small molecule,
- binding constant,
- affinity capillary Electrophoresis,
- microdialysis membrane
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