Citation: Xin Ya HE, Da Zhi LI, Ai Ye LIANG, Bing Cheng LIN. Evaluation of The Interaction between Netropsin and Double Stranded DNA by Capillary Zone Electrophoresis[J]. Chinese Chemical Letters, ;2003, 14(3): 304-307. shu

Evaluation of The Interaction between Netropsin and Double Stranded DNA by Capillary Zone Electrophoresis

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023

Received Date: 13 May 2002

Fund Project: This project is supported by NNSFC (grant number 20035010). The authors gratefully acknowledge this financial support.

Capillary zone electrophoresis (CZE) was applied to study the interaction between netropsin and a 14mer double stranded DNA (dsDNA). The binding constant of this interaction calculated from Scatchard plot was (1.07±0.10)×10⁵ (mol/L)^-1. The binding stoichiometry was 1:1. The use of polyacrylamide coated capillary showed better effect in the analysis of DNA than noncoated capillary.
- Interaction,
- netropsin,
- DNA,
- capillary zone electrophoresis
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