Citation: Ji ZHANG, Chun Sheng LIU, Yi Zhong ZHANG, Chang Jiu ZHANG, Li YE, Tong Hui ZHOU. GAS CHROMATOGRAPHIC-MASS SPECTROMETRIC ANALYSIS OF ANABOLIC STEROIDS IN HUMAN URINE[J]. Chinese Chemical Letters, ;1990, 1(2): 143-146. shu

GAS CHROMATOGRAPHIC-MASS SPECTROMETRIC ANALYSIS OF ANABOLIC STEROIDS IN HUMAN URINE

a Institute of Materia Medica, 1 Xian Nong Tan Street, Beijing 100050, P.R.China;
b National Research Institute of Sports Medicine, 11 Tiyuguan Road, Beijing 100061

Received Date: 15 December 1989

A new GC/MS method for detection and identification of 19 anabolic steroids in human urine is presented.The procedure involves adsorption and isolation on a macroporous XAD-2 resin,enzymatic hydrolysis,alkaline extraction,derivatization,GC separation and MS detection.Gas chromatographic-mass spectrometric data illustrate artifacts arising from enzymatic hydrolysis of steroid glucuronides and the structural characterization of their metabolites.Using this method,metabolic studies of these steroids in human urine were made after their ingestion by normal and healthy male volunteers.This method was proven to be suitable for large-scale routine analysis of anabolic steroids and was used successfully in passing the doping control test held by the Medical Commission of the International Olympic Committee.
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