Citation: Hua ZHANG, Wen Rui JIN. Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection[J]. Chinese Chemical Letters, ;2003, 14(9): 952-954. shu

Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection

Hua ZHANG ^1, ,
Wen Rui JIN ²

1.
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100;
2.
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100

Received Date: 11 September 2002

Fund Project: This project was supported by the National Natural Science Foundation of China, the State Key Laboratory of Microbial Technology, Shandong University.

A novel approach for analysis of amino acids in individual erythrocytes was established.In this method, the derivatization reagent was introduced into the living cells by electroporation.After derivatization, the amino acids in a single cell were determined by capillary electrophoresiswith laser-induced fluorescence detection.
- Capillary electrophoresis,
- laser-induced fluorescence detection,
- single cell analysis,
- amino acid
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