Citation: Zhi Xin WANG, Yi CHEN, Qing GUO. Key Conditions in Capillary Electrophoresis of Amino Acids with Indirect Ultraviolet Detection[J]. Chinese Chemical Letters, ;2000, 11(9): 803-806. shu

Key Conditions in Capillary Electrophoresis of Amino Acids with Indirect Ultraviolet Detection

Center for Molecular Science, Institute of Chemistry, The Chincse Academy of Sciences, Beijing 100080

Received Date: 20 March 2000

Fund Project: Thanks for the financial support of the National Natural Science Foundation of China (No.29635020) and the Chinese Academy of Sciences (No.KJ951-A1-507).

Key factors for capillary electrophoresis of amino acids with indirect ultraviolet detection were investigated.Buffer compositions and additives were found to be influential as expected, while buffer PH was shown to be particularly critical to the separation and detection. The effect of pH also depended on the electrophoretic mode used.Ways to obtain recurring and selective separations were suggested.
- Amino acids,
- capillary electrophoresis,
- indirect ultraviolet detection
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