Citation: Guang Ya XIANG, Heiner ECKSTEIN. Enzymatic Synthesis of a CCK-8 Tetrapeptide Fragment[J]. Chinese Chemical Letters, ;2004, 15(7): 768-770. shu

Enzymatic Synthesis of a CCK-8 Tetrapeptide Fragment

Guang Ya XIANG ^1, ,
Heiner ECKSTEIN ²

1.
Pharmacy School of Tongji Medical College, HUST, Wuhan 430030;
2.
Institute of Organic Chemistry, University of Tübingen, Tübingen, D-72076, Germany

Received Date: 11 July 2003

Fund Project: This work was supported by a grant from the Science and Research Foundation of the Ministry of Education (No.2003-406).

The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt is reported. It was synthesized by coupling Phac-Met-OEt with Gly-OMe·HCl, Trp-OMe and Met-OEt successively, catalyzed by α-chymotrypsin, papain and α-chymotrypsin respectively. The results of FAB-MS showed that the products had the correct molecular mass.
- CCK-8,
- enzymatic method,
- α-chymotrypsin,
- papain
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