Citation: Jun Feng SONG, Jian Xiu WANG, Wei GUO, Min Ren ZHAO. New Polarographic Immunoassay Using Copper Ion as Catalyst for Conversion of Substrate Instead of Natural Enzyme[J]. Chinese Chemical Letters, ;1996, 7(10): 922-923. shu

New Polarographic Immunoassay Using Copper Ion as Catalyst for Conversion of Substrate Instead of Natural Enzyme

Institute of Electroanalytical Chemistry Northwest University Xi'an 710069

Received Date: 2 April 1996

Fund Project: This paper was supported by the National Natural Science Foundation of China.

A new electrochemical immunoassay based on catalytic conversion of substrate by labeled metal ion and polarographic detection of generated product was developed.In this immunoassay, copper ion was used A catalyst for the conversion of substrate to substitute natural enzyme.Copper ion was labeled to antigens such as Human serum albumin( HSA ), Diphtheria Toxoid ( DT ), Tetanus Toxoid ( TT ) through bifunctional chelating agent diethylenetriamine pentaacetic acid ( DTPA ).After heterogeneous competitive immune reaction, the labeled copper ion was released by acidification, and then catalyzes chemically the conversion of substrate o-phenyldiamine ( OPD ) to electroactive product 2, 3-diaminophenazine ( DAP ).The product DAP was detected by using linearsweep polarography.The sensitivity of the proposed assay for the determination of HSA, DT and TT is superior to those of previous one based on direct detection of the labeled metal ion.
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