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Citation:
Jin YAN, Jie Bo MI, Wen Bao CHANG. Study of Immunoassay Methods for Recombinant Human Erythropoietin (rhEPO) Using Competitive ELISA[J]. Chinese Chemical Letters,
;2004, 15(8): 939-942.
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Two different immunoassay methods, competitive indirect enzyme-linked immuno-sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO.The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively.The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.
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