Citation: Ye Hua SHEN, Quan BAI, Yang Jun ZHANG, Yin Mao WEI, Hai Bo WANG, Xing Du GENG. A Novel Method for Diminishing Protein Aggregation during Denatuaration Process[J]. Chinese Chemical Letters, ;2006, 17(3): 395-398. shu

A Novel Method for Diminishing Protein Aggregation during Denatuaration Process

Institute of Modern Separation Science, The Key Lab of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an 710069

Received Date: 24 August 2005

Fund Project: These projects were supported by the National Natural Science Foundation of China (No. 39880003 and 20175016).

The addition of packing material for high performance hydrophobic interaction chrornatograghy (HPHIC) into the denaturant solution to prevent, or depress protein aggregation in the denatuaration process is presented. The renaturation of α-chymotrypsin (α-Chy)denatured with guanidine hydrochloride (GuHCl) solution indicated that renaturation efficiency can be enhanced from 36.1% to 59.0% by this new method. The structure of the ligand linking of HPHIC packings is also important for the protein renaturation.
- Protein renaturation,
- α-chymotrypsin,
- hydrophobic interaction chromatography
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