Citation: ZHi Qiang WANG, Gong ZHANG, Qi Min LIU, Jia Zan NI. FLUORESCENCE SPECTROSCOPY STUDIES ON TIIE ACTION OK RARE EARTHS WITH BOVINE SERUM ALBUMIN IN MULTIMOLECULAR SYSTEM[J]. Chinese Chemical Letters, ;1995, 6(10): 879-882. shu

FLUORESCENCE SPECTROSCOPY STUDIES ON TIIE ACTION OK RARE EARTHS WITH BOVINE SERUM ALBUMIN IN MULTIMOLECULAR SYSTEM

Laboratory of Rare Eare Chemistry and Physics, Changchun Institute Instituteof Applied Chemistry, Chinese Academy of Sciences, Changchun 130022

Received Date: 10 March 1995

Fund Project: Project supported by National Natural Science Foundation of China.

In this paper,the interaction of tripositive terbium ions (Tb³⁺) with bovine serum albumin (DSA) has been investigated in the presence of some alkaline earth metals and citric acid (Cit) by using fluorescence spectroscopy.The results show that Tb³⁺,BSA and Cit can form ternary complex BSA·Tb₂·Cit₄ in μ=0.1mol/1 NaCl,pH 6.3 hexaniethylenetetramine buffer.Other rare earths are able to compete for the same binding site in BSA with Tb³⁺.The sequence of competition is Eu³⁺ > Pr³⁺ > Yb³⁺ > Gd³⁺ > La³⁺.Ca²⁺ and Mg²⁺ can not replace Tb bound to BSA.
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