Citation: Lian Sheng LING, Zhi Ke HE, Fang CHEN, Hua Shan ZHANG, Yun E ZENG. Study on Triplex DNA by Use of Molecular “Light Switch” Complex of Ru(phen)₂(dppx)²⁺[J]. Chinese Chemical Letters, ;2003, 14(3): 300-303. shu

Study on Triplex DNA by Use of Molecular “Light Switch” Complex of Ru(phen)₂(dppx)²⁺

Lian Sheng LING ^1,2, ,
Zhi Ke HE ^1,2 ,
Fang CHEN ^1,2 ,
Hua Shan ZHANG ^1,2 ,
Yun E ZENG ^1,2

1.
Department of Chemistry, Wuhan University, Wuhan 430072;
2.
Institute of Chemistry, the Chinese Academy of Sciences, Beijing 100080

Received Date: 8 May 2002

Fund Project: The authors acknowledge the support of Alexander von Humboldt Foundation, CAS K.C. Wong post-doctoral Research award fond and Natural Science Foundation of China for postdoctoral fellowship, Educational Ministry of China, the National Natural Science Foundation of China(29605001), Wuhan University and its Alumni Association.

A new method for the study of triplex DNA is established according the fluorescence enhancement of molecular "Light Switch" complex of Ru(phen)₂(dppx)²⁺ when it intercalate into triplex DNA. Because the fluorescence intensity of Ru(phen)₂(dppx)²⁺ bonded to triplex DNA is in the case higher than that bonded to duplex DNA in certain range of DNA concentration, the method is much more sensitive than other methods reported previously.
- Ru(phen)₂(dppx)²⁺,
- molecular "Light Switch",
- triplex DNA
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