Citation: Zhen Hua CHEN, Zhong Yi YU, Quan YIN, Li XU, Ti Ping HE, Rong Sen SHEN. THE REACTION OF CALCIUM WITH WAZO-1 AND ITS BIOANALYTICAL APPLICATION[J]. Chinese Chemical Letters, ;1996, 7(5): 459-462. shu

THE REACTION OF CALCIUM WITH WAZO-1 AND ITS BIOANALYTICAL APPLICATION

Zhen Hua CHEN ^a,b,c ,
Zhong Yi YU ^a,b,c ,
Quan YIN ^a,b,c ,
Li XU ^a,b,c ,
Ti Ping HE ^a,b,c ,
Rong Sen SHEN

a Dept. of Chemistry, Wuhan univ., Wuhan 430072;
b Dept, of Res. & Analysis of Materials, Wuhan Univ. of Technology, Wuhan 430070;
c Dept. of Human Biological Chemistry and Genetics, The Uhiv. of Texas Medical Branch. Galveston. Texas. USA

Received Date: 26 October 1995

Fund Project: This work was supported by the National Natural Science Foundation of China.

A selective new reagent Wazo-1 was prepared.This paper studied the properties of its fluorescence and UV-Spectrum.A new fluorescence method for the determination of calcium is developed.Wazo-1 reac■s with calcium.forms calcium complex (Ex=297nm;Em=370nm).The interference of Mg(Ⅱ) has been studied.The reagent has better selectivity to Ca(Ⅱ) in the presence of large amount of Mg(Ⅱ).Flucrescence method has-been used for the determination of microamounts of calcium in serum with new reagent Wazo-1.The results obtained are favourable.
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