Citation: Jing XU, Lan Hua ZHAO, Xin Guo WU, Hong Mei WANG, Ru Xiu CAI. New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry[J]. Chinese Chemical Letters, ;2006, 17(7): 949-952. shu

New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry

1.
College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072;
2.
Stuff of Chemistry, Yunyang Medical College, Shiyan 442000;
3.
College of Resources and Environmental Science, Wuhan University, Wuhan 430072

Received Date: 4 January 2006

Fund Project: The authors gratefully acknowledge financial support from the National Natural Science Foundation of China (No. 20275027 and No. 20377032).

A highly sensitive stopped-flow spectrophotometry kinetic method was proposed forquantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide (NADH). Phenoxyl radicals generated from as low as 1×10^-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10^-9mol/L.
- Phenoxyl radicals,
- moleculary probe,
- stopped-flow spectrophotometry
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