Citation: Chang Kui SUN, Yu Zhen ZHANG, Ming CHEN. A Novel β-1, 4-N, 6-O-Diacetylmuramidase from Streptomyces griseus and its Chemical Modification[J]. Chinese Chemical Letters, ;2006, 17(11): 1469-1472. shu

A Novel β-1, 4-N, 6-O-Diacetylmuramidase from Streptomyces griseus and its Chemical Modification

State Key Laboratory of Microbial Technology, Shandong University, Ji'nan 250100

Received Date: 23 March 2006

Fund Project: The authors were grateful to the National Natural Science Foundation of China (No. 30470050) and Natural Science Foundation of Shandong Province (No. z2005d02) for financial support.

A novel lysozyme named β-1, 4-N, 6-O-diacetylmuramidase R2 was purified and characterized from Streptomyces griseus. The molecular weight of the enzyme was determined by MALDI-TOF-MS as 23.5 kDa. The N-terminal amino acid sequence was DTSGVQGIDVSHWQG. Chemical modification of β-1, 4-N, 6-O-diacetylmuramidase R2 indicated that sulfhydryl group and carbamidine of arginine residues are not essential for the activity of the enzyme, but lysine residues and imidazole of histidine residues are essential for the activity. The number of essential tryptophan and carboxyl groups was found that only one tryptophan residue and three carboxyl groups in the active site.
- Chemical modification,
- β-1,4-N,6-O-diacetylmuramidase,
- lysozyme
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