Citation: Rong CHEN, Pin YANG, Chun Ying WEI. La³⁺ Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence[J]. Chinese Chemical Letters, ;2000, 11(8): 725-726. shu

La³⁺ Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence

Institute of Molecular Science, Shanxi University, Taiyuan 030006

Received Date: 2 February 2000

Binding of La³⁺ to Fura-2 can change 340/380 nm fluorescence intensity ratio. Whether La³⁺ cross ventricular cell membrane was detected by this fluorescent probe technique. Fura-2 loaded isolated guinea pig ventricular cells were exposed to 0.01-0.1mM extracellular Lanthanum ion concentration, 340nm/380 nm fluorescence ratio was not changed. Using calcium channel agonist BAY K8644, KCL (35mM) depolarization to open the voltage-dependent calcium channel (VDCC); Adrenoceptor agonist to excite adrenoceptor, 340/380 ratio was not changed, suggesting that La³⁺ can not enter guinea pig ventricular cells in this case.
- Ventricular cells,
- adrenaline,
- La³⁺,
- transmembrane,
- Fura-2,
- BAY K8644
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