Citation: Xiu Ming JIANG, Zhi Chun CHEN, Shao Ming YANG, Han Feng LIN, Xian Fu LIN. Method of Spectrophotometric Microanalysis Based on HRP/PET Self-assembly Film[J]. Chinese Chemical Letters, ;2004, 15(5): 547-550. shu

Method of Spectrophotometric Microanalysis Based on HRP/PET Self-assembly Film

1.
Chemistry Department of Zhejiang University, Hangzhou 310027;
2.
Chemistry Department of Zhengzhou Institute of Technology, Zhengzhou 450052

Received Date: 29 April 2003

Fund Project: This research has been supported by International Cooperation Project of Zhejiang Science and Technology Bureau (021106132).

Horseradish peroxidase monolayer was assembled on the surface of PET-CO₂^- substrate. The reaction kinetics of HRP/PET film and H₂O₂ in micro reactor was studied using improved spectrophotometer. The relative activity of self-assembly HRP/PET film still remains above 80% after storing for 150 days at 4℃. When applied to determination of H₂O₂ in sample, the recoveries of H₂O₂ are 96.5%~101.1%.
- Electrostatic self-assembly film,
- horseradish peroxidase (HRP),
- spectrophotometry,
- atomic force microscope (AFM)
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