Citation: Kui JIAO, Wei SUN, Hai Yu WANG. A New Voltammetric Enzyme Immunoassay System for the Detection of Alkaline Phosphatase[J]. Chinese Chemical Letters, ;2002, 13(1): 69-70. shu

A New Voltammetric Enzyme Immunoassay System for the Detection of Alkaline Phosphatase

Department of Applied Chemistry, Qingdao Institute of Chemical Technology, Qingdao 266042

Received Date: 12 October 2000

Fund Project: The work was supported by the National Natural Science Foundation of China (Grant No.20075013) and the Natural Science Foundation of Shandong Province (Grant No.Y98B06025).

A new voltammetric enzyme immunoassay system was investigated based on p-nitrophenyl phosphate (PNPP) as the substrate for alkaline phosphatase (ALP).PNPP is enzymatically hydrolyzed and the product p-nitrophenol (PNP) is detected by differential pulse voltammetry (DPV), which can be oxidized at+1.02 V (vs.Ag/AgCl) on bare glass carbon electrode (GCE).The conditions for enzymatic reaction and electrochemical detection were studied.According to this method, ALP can be detected with a detection limit of 2.8×10² mU/L and a linear range of 4.0×10²~1.0×10⁶ mU/L.
- Alkaline phosphatase,
- p-nitrophenyl phosphate,
- p-nitrophenol,
- differential pulse voltammetry,
- voltammetric enzyme immunoassay
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