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Citation:
Li Ming DU, Zhe Feng FAN, Jin Li QIAO, Jing Ping WANG. Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization[J]. Chinese Chemical Letters,
;2001, 12(11): 1007-1010.
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Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%~95.7% and the RSD is 1.2%~4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.
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Keywords:
- Sparfloxacin,
- HPLC,
- pre-column derivatization,
- human urine
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