Citation: WANG Lingzhi, YUAN Hong, FANG Shibi, JIANG Yingyan. STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES[J]. Chinese Journal of Polymer Science, ;1993, 11(4): 364-369. shu

STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES

Received Date: 23 July 1992

The properties of immobilized glucose oxidase (GOD) by the complexes of diethylaminoethyl cellulose(DEAEC) with different polymers, such as polymethylacrylic acid (PMAA), polyacrylic acid (PAA), polystyrene sulfonic acid (PSSA), polyvinylaleohol (PVA), polyethylene oxide (PEO) and styrene-maleic acid copolymer (PSMA) were investigated. The activity of immobilized GOD was obviously influenced by the component of the DEAEC complexes. The relative activity of the immobilized GOD reached to maximum and over 90% of the native GOD,when the DEAEC-PMAA DEAEC-PAA complexes were used as a carrier with the molar ratio of DEAEC and polyacid of about one.Michaelis constants(Km) of the immobilized enzymes of DEAEC-GOD-PMAA and DEAEC-GOD-PAA were determined to be 1.25 and 1.00,respectively.Moreover,the immobilized GOD has a good strage stability and cyclic life.
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