Citation: Yong YU, Pei Qing YING, Gang JIN. Competitive Adsorption between Bovine Serum Albumin and Collagen Observed by Atomic Force Microscope[J]. Chinese Chemical Letters, ;2004, 15(12): 1465-1468. shu

Competitive Adsorption between Bovine Serum Albumin and Collagen Observed by Atomic Force Microscope

National Microgravity Lab, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080

Received Date: 24 September 2003

Atomic force microscopy (AFM) was used to study the competitive adsorption between bovine serum albumin (BSA) and type Ⅰ collagen on hydrophilic and hydrophobic silicon wafers.BSA showed a grain shape and the type Ⅰ collagen displayed fibril-like molecules with relatively homogeneous height and width, characterized with clear twisting (helical formation). These AFM images illustrated that quite a lot of type Ⅰ collagen appeared in the adsorption layer on hydrophilic surface in a competitive adsorption state, but the adsorption of BSA was more preponderant than that of type Ⅰ collagen on hydrophobic silicon wafer surface. The experiments showed that the influence of BSA on type Ⅰ collagen adsorption on hydrophilic surface was less than that on hydrophobic surface.
- Atomic force microscope,
- protein competitive adsorption,
- collagen,
- bovine serum albumin
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