Citation: Hong Tao YAN, Xiao Yun ZHU. Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection[J]. Chinese Chemical Letters, ;2000, 12(2): 157-160. shu

Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection

Department of Chemistry Northwest University Xian 710069

Received Date: 15 June 1999

A new analytical method for the determination of urea-urease system based on biochemical reaction heat induced laser beam deflection is presented in this paper.With the method, the Michaelis constant (K_m) of urease and apparent inhibition constant (K_i) of some metal ion inhibitors were measured respectively.This method was also used for the quantitative determination of metal ions with satisfactory result.
- Urease, kinetic parameter, metal ions determination, laser beam deflection.
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