Citation: Xue Mei LUO, Wei Jiang HE, Yu ZHANG, Zi Jian GUO, Long Gen ZHU. Site-specifically Hydrolytic Cleavage of Oxidized Insulin B Chain With Cu(Ⅱ) Ion[J]. Chinese Chemical Letters, ;2000, 11(11): 951-954. shu

Site-specifically Hydrolytic Cleavage of Oxidized Insulin B Chain With Cu(Ⅱ) Ion

State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093

Received Date: 8 May 2000

Fund Project: This work was supported by the National Natural Science Foundation of China (Grant Nos. 29871017 and 29823001).

Electrospray mass spectrometry investigation shows that denatured oxidized insulin B chain can be selectively cleaved by simple Cu(Ⅱ) ion and the site of cleavage is at Gly8-Ser9 bond which is second amide bond left from His 10 in the sequence of oxidized insulin B chain.
- Oxidized insulin B chain,
- hydrolytic cleavage,
- electrospray mass spectrometry,
- Cu²⁺
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