Citation: . CHARACTERIZATION OF MOLECULAR MASS OF SIX WATER-SOLUBLE POLYSACCHARIDE -PROTEIN COMPLEXES FROM GANODERMA TSUGAE MYCELIUM*[J]. Chinese Journal of Polymer Science, ;2003, 21(3): 309-316. shu

CHARACTERIZATION OF MOLECULAR MASS OF SIX WATER-SOLUBLE POLYSACCHARIDE -PROTEIN COMPLEXES FROM GANODERMA TSUGAE MYCELIUM*

Received Date: 10 April 2002
Revised Date: 9 August 2002

Six water-soluble polysaccharide-protein complexes coded as GM1, GM2, GM3, GM4, GM5 and GM6 wereisolated from the mycelium of Ganoderma tsugae by extracting with 0.2 mol/L phosphate buffer solution at 25, 40 and80℃, water at 120℃, 0.5 mol/L aqueous NaOH solution at 25 and 65℃, consecutively. Their chemical components wereanalyzed by using IR, GC, HPLC and 13C-NMR, and some new results were obtained. The four samples GM1, GM2, GM3 and GM4 are heteropolysaccharide-prote in complexes, in which, α- (1→3) linked D-glucose is the major monosaccharide while galactose,mannose and ribose are the secondary ones.GM5 and GM6 are β-(1→3)-D-glucan-protein complexes.The protein content increased from 32% to 69% with the progress of isolation.Weight-average molecular mass Mw and the intrinsic viscosity [η] of the GM samples in 0.5mol/L aqueous NaCl solution at 25℃ were measured systematically by laser light scattering (LLS),size exclusion chromatography(SEC) combined with LLS,and viscometry.The Mw of GMI to GM6 are 35.5,46.8,58.9,41.6,3.3 and 22.0×104,respectively.The conformation and molecular mass of the two fractions of sample GM5 were characterized satisfactorily by SEC-LLS without further fractionation.
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