Citation: Jiao Ning WANG, Ji Cun REN. A New Immunoassay Method by Capillary Electrophoresis with Enhanced Chemiluminescence Detection[J]. Chinese Chemical Letters, ;2005, 16(6): 793-796. shu

A New Immunoassay Method by Capillary Electrophoresis with Enhanced Chemiluminescence Detection

Department of Chemistry and Chemical Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240

Received Date: 8 June 2004

Fund Project: This work was financially supported by the National Natural Science Foundation of China (No. 20271033), the key project of National Natural Science Foundation of China (No. 20335020), and Natural Science Foundation of Shanghai (No. 02ZA14057).

This paper described a new immunoassay method by capillary electrophoresis with enhanced chemiluminescence (CL) detection system based on luminol-hydrogen peroxide reaction catalyzed by horseradish peroxides (HRP). Using para-iodophenol as a CL enhancer, the detection limit of about 1×10^-12 mol/L for HRP was achieved, which corresponded to 1.32×10^-5 U/mL. In optimal conditions, the free HRP-labeled CA125 antibody (Ab^*) and the bound enzyme-labeled complex (Ab^*-Ag) were well separated by capillary electrophoresis within 4 min.The assay was successfully used to determine the contents of CA125 in human sera, which were associated with ovarian cancer, and the recoveries of the standard addition experiments were 96 to 109%.
- Capillary electrophoresis,
- chemiluminescence detection,
- CA125,
- immunoassay
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