Citation: Hou Ping HUANG, Ru Xiu CAI, Yu Min DU, Yun E ZENG. LACCASE-BASED MICELLAR ENHANCED SPECTROFLUORIMETRIC DETERMINATION OF L-ASCORBIC ACID[J]. Chinese Chemical Letters, ;1995, 6(3): 235-238. shu

LACCASE-BASED MICELLAR ENHANCED SPECTROFLUORIMETRIC DETERMINATION OF L-ASCORBIC ACID

Department of Chemistry, Department of Environment Science, Wuhan University, Wuhan, 430072

Received Date: 17 October 1994

An automated flow-injection-stopped-flow micellar enhanced Spectrofluorimetric method for determination of L-ascorbic acid(AA)is described.The method is based on the interference of laccase by AA.causing a decrease in the rate of the laccase-catalysed fluorescence reaction in the presence of Brij-35.The resulting change of fluorescence intensity at em 530nm(ex 430nm)is proportional to the concentration of AA with the linear range over 0.2 to 2.0μg/ml.The significant interference from ferric and cupric ions can be eliminated by addition of ED-TA.The method has been applied to the determination of AA in pharmaceuticals.the results agree well with the nominal values and those obtained by the reference method.The recovery of AA in pharmaceuticals are between 91.5% and 115.4%.
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