Citation: Li Bo NIE, Jian Rong CHEN, Yu Qing MIAO, Nong Yue HE. Gold Nanoparticle-based Layer-by-Layer Enhancement of DNA Hybridization Electrochemical Signal at Carbon Nanotube Modified Carbon Paste Electrode[J]. Chinese Chemical Letters, ;2006, 17(6): 795-798. shu

Gold Nanoparticle-based Layer-by-Layer Enhancement of DNA Hybridization Electrochemical Signal at Carbon Nanotube Modified Carbon Paste Electrode

1.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004;
2.
Key Laboratory of Green-Packaging and Application of Bionanotechnology of Hunan Province, Hunan University of Technology, Zhuzhou 412008;
3.
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096

Received Date: 28 November 2005

Fund Project: 90406016), the Doctoral Program of Higher Education for Funding 20050286014, and Hunan Provincial Natural Science Foundation (05jj40053).This work was financially supported by the National Natural Science Foundation of China (No. 60571032

Colloid gold nanoparticle-based layer-by-layer amplification approach was applied to enhance the electrochemical detection sensitivity of DNA hybridization at carbon nanotube modified carbon paste electrodes (CNTPEs). Streptavidin was immobilized onto the surface of CNTPEs, and the conjugation of biotin labeled target oligonucleotides to the above immobilized streptavidin was performed, followed by the hybridization of target oligonucleotides with the gold nanoparticle-labeled DNA probe and then the layer-by-layer enhanced connection of gold nanoparticles, on which oligonucleotides complementary to the DNA probe were attached, to the hybridization system. The differential pulse voltammetry (DPV) signal of total gold nanoparticles was monitored. It was found that the layer-by-layer colloidal gold DPV detection enhanced the sensitivity by about one order of magnitude compared with that of one-layer detection. One-base mismatched DNA and complementary DNA could be distinguished clearly.
- Carbon nanotubes,
- carbon paste electrode,
- gold nanoparticles,
- layer-by-layer amplifycation,
- sensitivity
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