Citation: ZHUO Renxi, HE Feng, LIU Lijian, XU Mianyi. THERMAL ACTIVATION OF IMMOBILIZED PAPAIN*[J]. Chinese Journal of Polymer Science, ;1998, 16(2): 142-146. shu

THERMAL ACTIVATION OF IMMOBILIZED PAPAIN*

Received Date: 25 March 1997

Papain (Papainase, EC 3.4.22.2) was immobilized on porous silica beadsby cross linking with glutaraldehyde. The thermal activation of this immobilized papainin aqueous system was found at a temperature range from 50 to 90℃. The higher thetemperature, the more active the immobilized papain will possess. At the same time,the durability of the immobilized papain on heating was greatly improved. The effect ofadditives and salts on the activity of the immobilized papain were also studied. The results showed that the additives and some of the salts studied could markedly enhance the activity of the immobilized papain at elevated temperature.
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