Citation: Nong Yue HE, Hui Shi GUO, Di YANG, Chun Rong GU, Zhi Ping BIAN, Wen Hui WAN, Ji Nan ZHANG. Application of Nanogoid Probe Coupled with Silver Enhancement in Rapid cTnI Colorimetric Immunoassay[J]. Chinese Chemical Letters, ;2005, 16(12): 1625-1628. shu

Application of Nanogoid Probe Coupled with Silver Enhancement in Rapid cTnI Colorimetric Immunoassay

1.
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096;
2.
Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029

Received Date: 21 March 2005

Fund Project: This work was financially supported by the fund of "135" key laboratory of Jiangsu province, the high-tech research program of Jiangsu province, the Chinese Post Doctoral Science Foundation, the National Natural Science Foundation of China (No.60571032), and the Trans-Century Training Programme Foundation for the Talents by the Ministry of Education of China.

A rapid, inexpensive, reliable, and flexible quantitative immunoassay for cardiac troponin Ⅰ (cTnI) based on the concepts of one-step dual monoclonal antibody "sandwich" principle. The low density protein array, the nanogold probe, and the silver enhancement on the gold particle were provided. The whole detection procedure of the assay could be fulfilled within 40 min with the pretreated colloidal gold-labeled detection antibody and supporting substrate.The assay showed good specific response to cTnI with very low cross-reactivity ratio to the skeletal isoforms of troponin Ⅰ (sTnI), cardiac troponin T (cTnT), and myoglobin. 588 serum samples were assayed simultaneously by enzyme-linked immuno sorbent assay (ELISA) and this colloidal gold method to test the validity of the method and the data were analyzed using the statistical package SPSS version 11.0 (SPSS Inc.). There was no significant difference between these two assays (P=0.66>0.05). The agreement between this method (≥ or <0.3 ng/mL) and ELISA was 86%.
- Cardiac troponin Ⅰ,
- protein array,
- nanogold particles,
- silver enhancement,
- rapidquantitative immunoassay
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