Citation: Yun Xia WANG, Fang ZHANG, Jing LIANG, Hua LI, Ji Lie KONG. Determination of Enantiomeric Compositions of Tryptophan by Chemometric Analysis of the Fluorescence Spectra of Bovine Serum Albumin Receptor-ligand Mixtures[J]. Chinese Chemical Letters, ;2006, 17(12): 1599-1602. shu

Determination of Enantiomeric Compositions of Tryptophan by Chemometric Analysis of the Fluorescence Spectra of Bovine Serum Albumin Receptor-ligand Mixtures

Yun Xia WANG ^1,2, ,
Fang ZHANG ³ ,
Jing LIANG ³ ,
Hua LI ³ ,
Ji Lie KONG ¹

1.
Department of Chemistry, Fudan University, Shanghai 200433;
2.
Department of Chemistry, Northwest University, Xi'an 710069;
3.
Institute of Analytical Science, Northwest University, Xi'an 710069

Received Date: 21 September 2006

Fund Project: This work was supported by NNSFC (No. 20335040, 20525519, 20605005) and TRAPOYT.

In this work, a novel method was constructed to determine the enantiomeric composition of tryptophan (Trp) by bovine serum albumin (BSA) based on the fluorescence spectra of the receptor-ligand mixtures coupled with partial least squares (PLS-1) analysis. As a result the enantiomeric composition of Trp was accurately determined.
- Bovine serum albumin,
- tryptophan,
- PLS-1,
- fluorescence spectrophotometry
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