ZEROING ANISOTROPY SPECTROFLUOROMETRY

A new method of zeroing anisotropy spectrofluorometry has been developed. When a mixture of two components is determined by anisotropy spectrofluorimetric method, and the excitation wavelength is selected at the intersection points of the apparent excitation spectra corresponding to I_⊥ and I_// for the component A, then its anisotropy values are zero, and the measured anisotropy values can thus be used for the determination of the component B & vice versa. This method had been used for the simultaneous determination of a binary mixture of rhodamine B and pyronine G, and the determination of rhodamine B in a ternary mixture of rhodamine 6G, rhodamine S and rhodamine B using the common intersection points of I_⊥ and I_// excitation spectra of rhodamine 6G and rhodamine S as the excitation wavelength. The results are quite satisfactory.