Citation: Jin Mao YOU, Zong Qin RUAN, Jing Wu KANG. Studies on Synthesis of Stationary Phase Containing Nicotinamide Adenine Dinucleotide (NAD) Bonded to Phospholipid-Coated Aminated Silica for HPLC[J]. Chinese Chemical Letters, ;1997, 8(3): 243-246. shu

Studies on Synthesis of Stationary Phase Containing Nicotinamide Adenine Dinucleotide (NAD) Bonded to Phospholipid-Coated Aminated Silica for HPLC

Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, 730000

Received Date: 21 October 1996

Recently biospecific affinity chromatography has been widely used for the separation and purification of various enzymes and nucleic acids.In this paper,a series of synthetic reactions of solid-liquid phase were carried out on silica surface,using a macroporous (30μm), microspherical silica (8μm) as the matrix and γ-aminopropyltriethoxysilane as the activating agent,the nicotinamide adenine dinucleotide (NAD) was bonded through its amino groups to the carboxylic groups of linked phospholipid which was bonded covalently on aminated support.The bonded stationary phase has high thermal stability,and could be used to separate of nucleotides with good resolution.
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