Citation: Nong Yue HE, Jian Xin TANG, Song LI, Hong CHEN, An Cun ZHOU. In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane[J]. Chinese Chemical Letters, ;2005, 16(3): 397-400. shu

In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane

Nong Yue HE ^1, ,
Jian Xin TANG ¹ ,
Song LI ¹ ,
Hong CHEN ^1,3 ,
An Cun ZHOU ⁴

1.
Laboratory of Nanotechnology, Biomedicine and Devices, Zhuzhou Institute of Technology, Zhuzhou 412008;
2.
Chien-Shiung Wu Laboratory, Southeast University, Nanjing 210096;
3.
Life Science and Technology Department of Central South Forestry University, Changsha 410004;
4.
College of Hunan Traditional Chinese Medicine, Zhuzhou 412000

Received Date: 27 February 2004

Fund Project: This research is supported by the Trans-Century Training Programme Foundation for the Talents by the Ministry of Education of China and the Chinese Postdoctoral Science Foundation (2003).

Polypropylene microporous membranes were treated with plasma in a mixture of N₂ and H₂ (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstratedthe success of grafting amino groups. The density of the polar amino groups on the membrane surface is about 0.59 μmol/cm². The as-treated membranes were successively applied to the in situ synthesis of oligonucleotides and an average coupling yield was more than 98%. The surface feature of the treated membrane is suggested to be responsible for its advantage over a glass slide.
- Plasma,
- grafting,
- amino group,
- in situ synthesis of oligonucleotides,
- coupling efficiency,
- polypropylene microporous membrane.
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