Citation: Ke Qiang DING, Zhen Bin JIA, Qing Fei WANG, Juan BAI, Ru Ting TONG, Xin Kui WANG, Hui Bo SHAO. Impedance Characterization of Adsorption Process of Calmodulin on Au Substrate and its Combination with Ca²⁺[J]. Chinese Chemical Letters, ;2002, 13(4): 351-354. shu

Impedance Characterization of Adsorption Process of Calmodulin on Au Substrate and its Combination with Ca²⁺

1.
Institute of Coal Chemistry, Chinese Academy of Science, Taiyuan 030001;
2.
Department of Chemistry, Hebei Teachers University, Shijiazhuang 050016;
3.
Department of Chemistry, Capital Normal University, Beijing 100037

Received Date: 27 June 2001

Fund Project: This work is supported by the NNSFC (29973026) and Beijing Natural Science Foundation (2992007).

In this paper,the adsorption process of calmodulin (CaM) on Au substrate was first investigated with electrochemical impedance spectroscopy (EIS) method.The result reveals that the adsorption of the protein-calmodulin contains two steps,i.e.,one short quick step followed by a slow one.The complexation of calmodulin with Ca²⁺ was also first probed using EIS technique,in which the complexation of CaM with Ca²⁺ could be reflected by the change of apparent membrane capacitance(C_app) clearly.In all above measurements,a redox couple Fe(CN)₆^3-/ Fe(CN)₆^4- was used as probing-pin to reflect all the changes occurring in the above process.Our work suggests that some biological processes of CaM could be studied using EIS method conveniently.
- Calmodulin(CaM),
- adsorption behavior,
- Au substrate,
- complexation,
- electrochemical impedance spectroscopy
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