Synthesis of a new ratiometric emission Ca2+ indicator for in vivo bioimaging

Qiao-Ling Liu Meng Fan Wei Bian Shao-Min Shuang Chuan Dong

Citation:  Qiao-Ling Liu, Meng Fan, Wei Bian, Shao-Min Shuang, Chuan Dong. Synthesis of a new ratiometric emission Ca2+ indicator for in vivo bioimaging[J]. Chinese Chemical Letters, 2013, 24(2): 156-158. shu

Synthesis of a new ratiometric emission Ca2+ indicator for in vivo bioimaging

    通讯作者: Chuan Dong,
摘要: A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized. The indicator exhibits a highly selective ratiometric emission response to Ca2+ over other metal cations and a large Stokes shift of 202 nm. Moreover, its practical cell imaging capability for intracellular Ca2+ in the resting-and dynamic-state has been demonstrated in human umbilical vein endothelial cells using a confocal laser scanning microscope.

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    1. [1] (a) M.J. Berridge, M.D. Bottman, P. Lipp, Calcium-a life and death signal, Nature 395 (1998) 645-648;[1] (a) M.J. Berridge, M.D. Bottman, P. Lipp, Calcium-a life and death signal, Nature 395 (1998) 645-648;

    2. [2]

      (b) M. Nedergaard, A. Verkhratsky, Calcium dyshomeostasis and pathological calcium signalling in neurological diseases, Cell Calcium 47 (2010) 101-102;(b) M. Nedergaard, A. Verkhratsky, Calcium dyshomeostasis and pathological calcium signalling in neurological diseases, Cell Calcium 47 (2010) 101-102;

    3. [3]

      (c) N. Steinckwich, V. Schenten, C. Melchior, et al., An essential role of STIM1, Orai1, and S100A8-A9 proteins for Ca2+ signaling and FcγR-mediated phagosomal oxidative activity, J. Immunol. 186 (2011) 2182-2191.(c) N. Steinckwich, V. Schenten, C. Melchior, et al., An essential role of STIM1, Orai1, and S100A8-A9 proteins for Ca2+ signaling and FcγR-mediated phagosomal oxidative activity, J. Immunol. 186 (2011) 2182-2191.

    4. [2] (a) R.D. Burgoyne, Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signaling, Nat. Rev. Neurosci. 8 (2007) 182-193;[2] (a) R.D. Burgoyne, Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signaling, Nat. Rev. Neurosci. 8 (2007) 182-193;

    5. [5]

      (b) P. Vito, E. Lacana, L. D0Aamio, Interfering with apoptosis: Ca2+-binding protein ALG-2 and Alzheimer's disease gene ALG-3, Science 271 (1996) 521-525.(b) P. Vito, E. Lacana, L. D0Aamio, Interfering with apoptosis: Ca2+-binding protein ALG-2 and Alzheimer's disease gene ALG-3, Science 271 (1996) 521-525.

    6. [3] (a) K. Satoh, T. Matsu-Ura, M. Enomoto, et al., Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells, J. Biol. Chem. 286 (2011) 20591-20599;[3] (a) K. Satoh, T. Matsu-Ura, M. Enomoto, et al., Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells, J. Biol. Chem. 286 (2011) 20591-20599;

    7. [7]

      (b) K.J. Buckler, Effects of exogenous hydrogen sulphide on calcium signalling, background (TASK) K channel activity and mitochondrial function in chemoreceptor cells, Pflugers Arch. 463 (2012) 743-754;(b) K.J. Buckler, Effects of exogenous hydrogen sulphide on calcium signalling, background (TASK) K channel activity and mitochondrial function in chemoreceptor cells, Pflugers Arch. 463 (2012) 743-754;

    8. [8]

      (c) T.P. Collins, D.A. Terrar, Ca2+-stimulated adenylyl cyclases regulate the L-type Ca2+current in guinea-pig atrial myocytes, J. Physiol. 590 (2012) 1881-1893.(c) T.P. Collins, D.A. Terrar, Ca2+-stimulated adenylyl cyclases regulate the L-type Ca2+current in guinea-pig atrial myocytes, J. Physiol. 590 (2012) 1881-1893.

    9. [4] R.Y. Tsien, New calcium indicators and buffers with high selectivity against magnesium and protons: δesign, synthesis, and properties of prototype structures, Biochemistry 19 (1980) 2396-2404.[4] R.Y. Tsien, New calcium indicators and buffers with high selectivity against magnesium and protons: δesign, synthesis, and properties of prototype structures, Biochemistry 19 (1980) 2396-2404.

    10. [5] X.Z. Guo, X.M. Wang, Y.K. Du, et al., Synthesis and nonlinear optical properties of new quadrupolar chromophores, Chin. Chem. Lett. 16 (2005) 597-600.[5] X.Z. Guo, X.M. Wang, Y.K. Du, et al., Synthesis and nonlinear optical properties of new quadrupolar chromophores, Chin. Chem. Lett. 16 (2005) 597-600.

    11. [6] G. Grynkiewicz, M. Poenie, R.Y. Tsien, A new generation of Ca2+ indicators with greatly improved fluorescence properties, J. Biol. Chem. 260 (1985), 3440-2450.[6] G. Grynkiewicz, M. Poenie, R.Y. Tsien, A new generation of Ca2+ indicators with greatly improved fluorescence properties, J. Biol. Chem. 260 (1985), 3440-2450.

    12. [7] (a) A.M.B. Reeves, E. Shigetomi, B.S. Khakh, Bulk loading of calcium indicator dyes to study astrocyte physiology: key limitations and improvements using morphological maps, J. Neurosci. 31 (2011) 9353-9358;[7] (a) A.M.B. Reeves, E. Shigetomi, B.S. Khakh, Bulk loading of calcium indicator dyes to study astrocyte physiology: key limitations and improvements using morphological maps, J. Neurosci. 31 (2011) 9353-9358;

    13. [13]

      (b) M. Kamiya, K. Johnsson, Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore, Anal. Chem. 82 (2010) 6472-6479;(b) M. Kamiya, K. Johnsson, Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore, Anal. Chem. 82 (2010) 6472-6479;

    14. [14]

      (c) M. Collot, C. Loukou, A.V. Yakovlev, et al., Calcium rubies: a family of redemitting functionalizable indicators suitable for two-photon Ca2+ imaging, J. Am. Chem. Soc. 134 (2012) 14923-14931.(c) M. Collot, C. Loukou, A.V. Yakovlev, et al., Calcium rubies: a family of redemitting functionalizable indicators suitable for two-photon Ca2+ imaging, J. Am. Chem. Soc. 134 (2012) 14923-14931.

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  • 收稿日期:  2012-11-04
  • 网络出版日期:  2012-12-05
通讯作者: 陈斌, bchen63@163.com
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