Citation: Jin Mao YOU, Xing Jun FAN, Qing Yu OU, Qing Cun ZHU. HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride[J]. Chinese Chemical Letters, ;1998, 9(3): 281-285. shu

HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride

a Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences 730000;
b Department of Chemistry, Qufu Normal University, Qufu, Shandong 273165

Received Date: 10 October 1997

A sensitive HPLC method for the detection of amino acids with pre-column fluorescence derivatization with carbazole-9-yl-acetyl chloride has been developed. This method, in conjunction with a multi-gradient program, offers baseline resolution of the common CRAC-amino acids from a linear acetonitrile gradient. Separation of amino acid derivatives was obtained on a reversed-phase C₁₈ column. Derivatization and chromatographic conditions were optimized. Amino acid derivatives were eluted in 40 min with good reproducibility. The relative standard deviations (n=6) at an analytical concentration of 100 pmol are <4%. The methods described are also suitable for the analysis of amino acids in different biological samples.
- liquid chromatography,
- amino acids,
- fluorescence derivatization,
- carbazole-9-yl-acetylchloride
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