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Citation: WANG Xiang-Hong, XIE Bing. Spectrophotometric Determination of Inosine with Brilliant Cresyl Blue[J]. Chinese Journal of Applied Chemistry, ;2009, 26(8): 999-1001. shu

Spectrophotometric Determination of Inosine with Brilliant Cresyl Blue

WANG Xiang-Hong ^, ,
XIE Bing

1.
Department of Chemistry, Yangtze Normal university, ChongQing 408100

Corresponding author: WANG Xiang-Hong,
Received Date: 15 August 2008
Available Online: 23 October 2008
Fund Project: 重庆市教委科研项目(KJ081303,KJ071301) (KJ081303,KJ071301)

In a pH=6.9 BR buffer medium,brilliant cresyl blue reacted with inosine to form ion-association complex.The maximum fading wavelength was located at 630 nm.The fading intensity is proportional to the concentration of inosine.The concentration of inosine in a range of 0.18×10^-6~6.0×10^-6 g/mL obeys Beer's Law.The correlation coefficient is 0.999 3,the molar absorptivity is 1.13×10⁴ L/(mol·cm),the detection limit is 53.5×10^-9 g/mL.The method is simple,accurate and sensitive,and it was applied to the determination of inosine in tablet samples with satisfactory results.
- Inosine,
- spectrophotometric,
- Brilliant Cresyl blue
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