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Citation: LI Nan, WANG Lin-Lin, LIU Hai-Yan, ZHANG Jie, LIU Jun, WANG Ying-Hua. Determination of Hypoxanthine,Xanthine and Adenine Extracts in Four Animal Drugs by HPLC[J]. Chinese Journal of Applied Chemistry, ;2009, 26(8): 996-998. shu

Determination of Hypoxanthine,Xanthine and Adenine Extracts in Four Animal Drugs by HPLC

1.
College of Chemistry, Jilin University, Changchun 130012

Corresponding author: WANG Ying-Hua,
Received Date: 7 July 2008
Available Online: 28 December 2008

Hypoxanthine,xanthine and adenine in some animal drug materials were determined by means of HPLC with methanol/water as the mobile phase and a UV detector.This process of sample preparation was improved and the conditions of HPLC were also optimized.The detection limits of hypoxanthine,xanthine and adenine were 0.012×10^-6,0.041×10^-6,0.042×10^-6 g/mL,respectively.The intra-day precisions(RSD) of hypoxanthine,xanthine and adenine were less than 4.1%,7.3% and 4.5%,respectively.The inter-day precisions(RSD) of hypoxanthine,xanthine and adenine were less than 5.8%,2.9% and 3.1%,respectively.The recovery rates of the four samples with added specimen were in a range of 94% to 107%.
- high performance liquid chromatography,
- ultrasound extraction,
- purine,
- animal drugs
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