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Citation: CUI Feng-Ling, QIN Li-Xia, LI Fang, Hao Er-Jun. Determination of Proteins in Biology Samples by Synchronous Fluorescence Technique with 5-Nitrosalicylic Acid as a Probe[J]. Chinese Journal of Applied Chemistry, ;2009, 26(6): 730-733. shu

Determination of Proteins in Biology Samples by Synchronous Fluorescence Technique with 5-Nitrosalicylic Acid as a Probe

1.
College of Chemistry and Environmental Science of Henan Normal University, Xinxiang 453007

Corresponding author: CUI Feng-Ling,
Received Date: 6 May 2008
Available Online: 20 July 2008
Fund Project: 国家自然科学基金(20673034) (20673034)高等学校博士学科点专项科研基金(20060476001) (20060476001)河南省青年骨干教师资助计划(200470) (200470)新乡市科技发展计划(07S038)资助 (07S038)

5-Nitrosalicylic acid can react with human serum albumin(HSA) to form a complex.Based on this,a new method for the determination of proteins by synchronous fluorescence spectra under simulated physiological conditions was established with 5-nitrosalicylic acid as a fluorescence probe.The spectral chara-cterization and the intensity of synchronous fluorescence were related to the value of Δλ,reaction medium and reaction temperature is.Experimental results show that under the optimum conditions,the synchronous fluorescence intensity of the system is in proportion to the concentration of HSA in a range of 3.2×10^-8~6.4×10^-6 mg/L.The detection limit is 1.17×10^-8 mol/L(n=11).The determination of proteins in human serum,urine and saliva samples is performed with satisfactory results,the recovery is within a range of 99.96%~100.04%.
- nitrosalicylic acid(NSA),
- human serum albumin,
- synchronous fluorescence spectroscopy,
- determination
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