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Citation: LI Run, ZHANG Wan-Kuan, NI Hang-Sheng, DAI Jun-Biao, WANG Hong, ZHOU Chun-Yu. Separation and Analysis DHA from Isochrysis Galbana[J]. Chinese Journal of Applied Chemistry, ;2001, 18(6): 454-456. shu

Separation and Analysis DHA from Isochrysis Galbana

1.
a Department of Chemistry, Tsinghua University, Beijing 100084;
2.
b Chinese Environmental Science Research Institute, Beijing

Received Date: 30 July 2000
Available Online: 1 February 2001

Isochrysis galbana is used as a new source of DHA (docosahexaenoic acid). The fatty acid of Isochrysis galbana was extracted by a mixture of CH₃Cl, CH₃OH and H₂O(1:2:0.8 in volume) and esterified by a mixture of BF₃ and C₂H₅OH(1:3 in volume), respectively. The esterification degree was detected with silica gel thin films. The quantitative analysis for DHA was carried out by GC and GC/MS techniques using ethyl ester of C₁₉ fatty acid as an internal standard. DHA in yield of 2.2% was obtained from algae isochrysis galbana.
- isochrysis galbana,
- docosahexaenoic acid,
- separation
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