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Citation: YUAN Chun-Tao, IANG Xian-Ming. Immobilization of Papain onto Poly(chitosan-co-acrylonitrile)[J]. Chinese Journal of Applied Chemistry, ;2002, 19(9): 862-865. shu

Immobilization of Papain onto Poly(chitosan-co-acrylonitrile)

YUAN Chun-Tao ^a,, ,
IANG Xian-Ming ^b

1.
a Department of Chemistry, Xiangtan Normal University, Xiangtan 411201;
2.
b Department of Chemistry and Chemical Engineering, Guilin

Corresponding author: YUAN Chun-Tao,
Received Date: 26 December 2001
Available Online: 10 May 2002
Fund Project:

Papain was immobilized on poly(chitosan-co-acrylonitrile). The properties of immobilized enzyme such as Michaels constant, thermal stability and half-working life were studied. The results showed that the matrix could greatly adsorb papain with thermal stability increased. The optimum immobilization temperature was 70℃. After treatment at 70℃ for 9 h, 91% activity of the immobilized papain was retained. In comparison with native enzyme the thermal stability of immobilized papain on poly(chitosan-co-acrylonitrile) was improved considerably. After at storage 4℃ for 70 d, no turbidness was observed in beer treated with poly(chitosan-co-acrylonitrile) immobilized papain.
- chitosan,
- graft copolymer,
- immobilized,
- papain,
- beer
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