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Citation: QI Chao, ZHAO Jian-Fei, LI Wei-Guo, DU Li, LIU Li-Yan. Study on the Wistar Rat Liver and Its Walker Tumor Chromatins by Fluorescence Lifetime and Calorimetric Measurements[J]. Chinese Journal of Applied Chemistry, ;2003, 20(12): 1135-1138. shu

Study on the Wistar Rat Liver and Its Walker Tumor Chromatins by Fluorescence Lifetime and Calorimetric Measurements

QI Chao ^a,b,, ,
ZHAO Jian-Fei ^a ,
LI Wei-Guo ^b ,
DU Li ^c ,
LIU Li-Yan ^b

1.
a Key Laboratory of Structural Biology, College of Life Science, University of Science and Technology of China, Hefei 230027;
2.
b Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun;
3.
c Department of Biochemistry, Quartermaster University of PLA, Changchun 130062

Corresponding author: QI Chao,
Received Date: 26 April 2003
Available Online: 28 October 2003
Fund Project: 国家自然科学基金(29701005) (29701005)中国科学院基础局重大项目(KJ951A150402)资助项目 (KJ951A150402)

The structures of rat liver chrotin and its walker tumoral chromatin have been studied by fluorescence lifetime, precision calorimetric determinations and energy transfer measurement. The results show that the component with ethidium bromide inserting DNA in tumoral chromatin(TC) increases by 4.86% than that in normal chromatin(NC). The formation enthalpy of the complex from ethidium bromide and DNA in TC is -15.38 kJ/mol and -13.25 kJ/mol for NC. The energy transfer efficiency between dansyl chloride and acridine orange in labeled TC is lower than that in labeled NC. The mean donor-acceptor distance is longer than that in labeled NC. These results denote that TC has a less rigid structure and higher genic activity, and can more easily form complex with ethidium bromide.
- chromatin,
- calorimetry,
- energy transfer,
- fluorescence lifetime
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