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Citation: GAO Feng, ZHU Ying-Gui, ZHANG Li, XIE Xiao-Juan, WANG Lun. Synthesis of a Fluorescence Reagent Bis(benzotriazo-1-le)-ethyldione Semicarbazone and Its Application in Determination of Proteins[J]. Chinese Journal of Applied Chemistry, ;2004, 21(4): 374-377. shu

Synthesis of a Fluorescence Reagent Bis(benzotriazo-1-le)-ethyldione Semicarbazone and Its Application in Determination of Proteins

1.
College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000

Corresponding author: WANG Lun,
Received Date: 4 June 2003
Available Online: 10 October 2003
Fund Project: 安徽省教育厅基金(2003kj144) (2003kj144)安徽省高校青年教师基金(2003jq104) (2003jq104)

A novel fluorescence reagent bis(benzotriazo-1-le)-ethyldione semicarbazone(BTES)was synthesized from 1-H-benzotriazole, biformyl and semicarbazide. The structure of the reagent was characterized by elemental analysis, IR spectrometry and ¹H NMR spectrometry. The reagent gave fluorescence emission at 342 nm in a KH₂PO₄-H₃PO₄ buffer solution(pH=4.0). A new method for the determination of trace protein has been developed based on the enhanced fluorescence emission in the presence of a certain amount of protein. Under optimal conditions the linear range concentration was at 08.0×10^-6g/mL and 1.0×10^-50.25g/mL, for bovine serum albumen and human serum albumen, respectively. The detection limit was 5.3×10^-9g/mL and 1.4×10^-9g/mL, respectively. The method has been tested on the synthesized samples with satisfactory results.
- bis(benzotriazo-le)-ethyldione-semicarbazone,
- protein,
- fluorespectrophotometry
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