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Citation: LI Lai-Sheng, HUANG Zhi-Bing, LIU Xu, XIE Bing, WANG Yu-Xiao. Study of Interaction of p-(N,N'-Dimethylaminomethyl)-calix[8]arene with DNA Using Methylene Blue as Spectroscopic Probe[J]. Chinese Journal of Applied Chemistry, ;2004, 21(10): 1011-1015. shu

Study of Interaction of p-(N,N'-Dimethylaminomethyl)-calix[8]arene with DNA Using Methylene Blue as Spectroscopic Probe

1.
The Center of Analysisand Testing, Nanchang University, Nanchang 330047

Corresponding author: LI Lai-Sheng,
Received Date: 1 December 2003
Available Online: 22 May 2004
Fund Project: 江西省教委基金资助项目(JJ2003-73-16) (JJ2003-73-16)江西省自然科学基金资助项目(9920024) (9920024)

The interaction of water soluble p-(N,N'-dimethylaminomethyl)calix\arene(CX8) with calf thymus DNA has been studied by UV-vis spectroscopy and fluorescence spectrophotometry using methylene blue(MB) as a probe. The effect of solution pH, ionic strength, common organic solvents and surfactants on the UV adsorption and fluorescence intensity were investigated. The results show that there is a strong electrostatic interaction between DNA and CX8. Scatchard curves indicated that the electrostatic interaction occurs between DNA and CX8, i.e. CX8 can compete for the electrostatic bonding sites of MB on DNA.
- p-(N,N'-dimethylaminomethyl)calix[8]arene,
- calf thymus DNA,
- methylene blue,
- UV-vis spectroscopy,
- fluorescence probe
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