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Citation: LONG Yun-Fei, JIANG Wen-Jun, CHEN Xiao-Ming, SU Jie-Shu, LI Ying. Determination of DNA by Resonance Light Scattering Method Using Phenothalein as a Sensitizer[J]. Chinese Journal of Applied Chemistry, ;2004, 21(11): 1187-1189. shu

Determination of DNA by Resonance Light Scattering Method Using Phenothalein as a Sensitizer

1.
a Institute of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201;
2.
b College of Chemistry, Xiangtan University, Xiangtan

Corresponding author: LONG Yun-Fei,
Received Date: 26 December 2003
Available Online: 10 June 2004
Fund Project: 湖南省自然科学基金(00JJY2084) (00JJY2084)湖南省教育厅基金(02C462)资助项目 (02C462)

Phenothalein-deoxyribonucleic acid(DNA)-cetyltrimethyl ammonium bromide(CTMAB) system has been studied by resonance light-scattering(RLS). In Tris-HCl buffer solution(pH=9.30), phenothalein and DNA react with CTMAB to form large particles of ion-association complex, which results in significant enhancement of RLS intensity. The spectral characteristics of RLS, the effective factors and optimum conditions of the reaction have been investigated. Under optimum conditions, the enhanced RLS intensity was proportional to the concentration of DNA in the range of 0.04 to 0.8 mg/L for fish sperm DNA(fsDNA) and 0.03 to 2.5 mg/L for calf thymus DNA(ctDNA). The detection limits were 12.0 μg/L and 17.0 μg/L for fsDNA and ctDNA, respectively.
- DNA,
- resonance light-scattering,
- cetyltrimethyl ammonium bromide,
- phenothalein,
- quantitative determination
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