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Citation: SUN Wei, HAN Jun-Ying, JIAO Kui, LU Lu-De. Linear Sweep Voltammetric Determination of Protein with Beryllon Ⅱ as Probe[J]. Chinese Journal of Applied Chemistry, ;2005, 22(3): 242-245. shu

Linear Sweep Voltammetric Determination of Protein with Beryllon Ⅱ as Probe

SUN Wei ^a,b,, ,
HAN Jun-Ying ^a ,
JIAO Kui ^a ,
LU Lu-De ^b

1.
a College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042;
2.
b College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing

Corresponding author: SUN Wei,
Received Date: 26 June 2004
Available Online: 30 August 2004
Fund Project: 中国博士后科学基金(2003033492) (2003033492)国家自然科学基金(20405008,20375020)资助项目 (20405008,20375020)

Beryllon Ⅱ interacts with protein to form a supermolecular complex in a pH 4.3 Britton-Robinson (B-R) buffer solution and produces a sensitive reduction peak at -0.48 V (vs.SCE) on the hanging mercury drop electrode.According to the decrease of the reduction peak current without the shifting of the peak potential of beryllon Ⅱ after the addition of human serum albumin (HSA) to the above solution,a new linear sweep voltammetric determination method for protein is proposed.Under the optimal electrochemical conditions,the reduction peak current of beryllon Ⅱ decreases linearly with the HSA concentration ranged from 5.0 to 50.0 mg/L and the linear regression equation is Δi"_p(nA)=49.83+3.65c(mg/L),γ=0.996 6.The method was applied to determine the content of HSA in human blood samples and the result was in good agreement with that obtained by the traditional Coomassie brilliant blue G-250 spectrophotometric method.The method was also used to determine bovine serum album in,bovine hemoglobin with satisfactory results.
- protein,
- beryllon Ⅱ,
- linear sweep voltammetry
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