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Citation: ZHAO Feng-Mei, WANG Hai-Tang, FU Yu-Bin. Enzyme-Catalyzed Synthesis and Characterization of Diacetylenic Glycerophosphatidylhydroxyethanol[J]. Chinese Journal of Applied Chemistry, ;2005, 22(5): 575-577. shu

Enzyme-Catalyzed Synthesis and Characterization of Diacetylenic Glycerophosphatidylhydroxyethanol

1.
a Chemistry & Pharmaceutical College, Henan University of Science and Technology, Luoyang 471003;
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b Luoyang Ship Materials Research Institute, Luoyang

Corresponding author: ZHAO Feng-Mei,
Received Date: 16 July 2004
Available Online: 30 October 2004
Fund Project: 船舶工业国防科技应用基础研究基金资助项目(01J.1.47) (01J.1.47)

Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds.The product structure was characterized by elemental analysis,IR,¹H NMR and ¹³C NMR.The effects of the reaction conditions on the enzyme-catalyzed reaction are discussed.The results show that the enzyme activity was greatly increased by adding SDS.The yield of the reaction was increased compared with that reported in the literature under the conditions of pH=5.6,temperature 30℃,Ca²⁺ 40 mmol/L,n(substrate):n(SDS)=(2:1),reaction time 8 h.The cost was lower.The melting point of the product was 69.5℃.
- phospholipase D,
- diacetylenic glycerophosphatidylcholine,
- diacetylenic glycerophosphatidyl-hydroxyethanol,
- enzyme catalyzed reaction
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