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Citation: LI Zhen-Zhong, JIANG Zhi-Liang, CHEN Yuan-Yuan, SUN Shuang-Jiao, ZHOU Su-Mei. The Spectral Feature of Protein-Cationic Surfactant Associated Particles and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, ;2005, 22(12): 1304-1307. shu

The Spectral Feature of Protein-Cationic Surfactant Associated Particles and Its Analytical Application

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a Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004;
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b Department of Basic Chemistry, Youjiang Medical College for Nationalities, Baise

Corresponding author: JIANG Zhi-Liang,
Received Date: 26 December 2004
Available Online: 28 May 2005
Fund Project: 广西自然科学基金(014403) (014403)

In the medium of NaOH-EDTA,cationic surfactant tetradecylmethylbenzylammonium chloride,(TDMBA),associaues with proteins,such as human serum albumin(HSA), bovine serum albumin(BSA),γ-globulin(γ-G) and ovalbumin(Ova),to form associated particles,which enhances the resonance scattering.There were 5 resonance scattering peaks at 340,400,420,470,and 520 nm,respectively,and the strongest one was at 470 nm after TDMBA was added to the HSA system.When the HSA association system was excited by the light at 280 nm,it exhibited a strong fluorescence peak at 470nm,while the fluorescence peak at 330 nm quenched with the addition of the associated particles.Under proper experimental conditions,the protein determination can be performed in the range of 0.168.0 mg/L for HSA,0.0816.0 mg/L for BSA,0.1616.0 mg/L for γ-G,and 0.660.0 mg/L for Ova.The detection limits are 0.01,0.01,0.01,0.06 mg/L for HSA,BSA,γ-G,and Ova respectively.This method was applied to the determination of the total proteins in human serum with satisfactory results.
- cationic surfactant,
- HSA,
- associated particle,
- resonance scattering spectrum
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