Citation:
LI Chun-Ying, ZHAO Chun-Jian, ZU Yuan-Gang. Simultaneous Determination of Solanesol and Coenzyme Q10 in the Extract of Tobacco(Nicotiana Tabacum L.) Leaves by HPLC[J]. Chinese Journal of Applied Chemistry,
;2006, 23(5): 524-527.
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The simultaneous determination of solanesol and coenzyme Q10 in the extract of tobacco(Nicotiana Tabacum L.) leaves by using reversed phase high performance liquid chromatography(RP-HPLC) method was established.The sample preparation process involved homogeneous extraction.The chromatogram was obtained on an HIQ SIL C18V column(4.6 mm×250 mm,5 μm) with V(acetonitrile):V(isopropanol)=8:7 as the mobile phase at a flow rate of 1.5 mL/min and 25℃.Diode array detection(DAD) was used and UV detection wave wavelength was set at 215 nm for solanesol and 275 nm for coenzyme Q10. The volume of injection sample was 20 μL.Under the chromatographic conditions,the recoveries of solanesol and coenzyme Q10 were(100.05±1.25)% and (99.97±1.36)% respectively.The method is simple,rapid,efficient and it is suitable to the determination of solanesol and coenzyme Q10 in the extract of tobacco leaves.
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Keywords:
- RP-HPLC,
- DAD,
- tobacco leaves,
- solanesol,
- coenzyme Q10
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