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Citation: LIU Ping, ZHU Ya-Qi, MA Jie, XUE Ying, WU Guo-Hua. Determination of Trace Penicillin in Milk By Self-made Hematein Modified Electrode[J]. Chinese Journal of Applied Chemistry, ;2006, 23(5): 519-523. shu

Determination of Trace Penicillin in Milk By Self-made Hematein Modified Electrode

LIU Ping ^a ,
ZHU Ya-Qi ^a ,
MA Jie ^a,, ,
XUE Ying ^b ,
WU Guo-Hua ^b

1.
a Department of Chemistry, Capital Normal University, Beijing 100037;
2.
b Institute for Nutrition and Food Safety, Center for Disease Control and Prevention of Beijing, Beijing

Corresponding author: MA Jie,
Received Date: 5 August 2005
Available Online: 16 October 2005
Fund Project: 北京市自然科学基金资助项目(6052002) (6052002)

Penicillinase,hematein and nano gold colloid were immobilized,as a modified membrane,on a plane Pt electrode by glutaraldehyde-bovine serum albumin.The membrane-modified electrode was used to construct amperometric electrochemical biosensors for the determination of trace penicillin in milk.The principle is based on the use of pH-sensitive hematein as the redox-active probe molecule.At a suitable constant potential,when the local pH on the electrode surface or in its promixity changes as a consequence of the penicillin hydrolyzation,the current flow resulting from the electrochemical reaction of hematein also changes to an extent,which is correlated to the substrate concentration.To characterize the interactions of enzyme with nano Au colloid and hematein,electrochemical impedance spectroscopy(EIS) was used.The linear detection range of the modified Pt electrode is from 2.81×10^-5 to 1.26×10^-2 mmol/L in PBS and from 5.61×10^-4 to 2.10×10^-3 mmol/L in milk.The response time is 4 s.The electrode can be used for at least 15 days with the perfect reproducibility.The life of the electrode is 30 days.
- amperometric electrochemical biosensor,
- hematein,
- penicillin
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