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Citation: ZHU Xian, SONG Hong-Li, CHENG Hong-Bin. HPLC Analysis of Amino Acid from Biomass Solid Waste Hydrolysis in Subcritical Water[J]. Chinese Journal of Applied Chemistry, ;2006, 23(9): 1052-1054. shu

HPLC Analysis of Amino Acid from Biomass Solid Waste Hydrolysis in Subcritical Water

1.
School of Evirn & Chemical Engineering, Department Chemical Engineering, Shanghai University, Shanghai 201800

Corresponding author: ZHU Xian,
Received Date: 16 October 2005
Available Online: 16 February 2006
Fund Project: 国家自然科学基金资助项目(50578091) (50578091)上海市教委第四期重点学科项目(B68-3) (B68-3)

The HPLC analysis of amino acids in subcrtical hydrolysate of the fish solid waste was set up.In this method,DNFB was used as derivatizing agent.The derivatizing reaction conditions were: pH>7,reaction temperature 60℃,and reaction time 1 h.The HPLC analysis conditions were:chromatographic column hypeoil ODS-C₁₈(100 mm×4.6 mm i.d.,5 μm);mobile phase: sodium acetate and acetonitrile-water;detection wavelength 360 nm,column temperature 45℃;linear gradient elution program.The recovery rate was 90.40%~110.80% and C_v was 0.91%~8.58%.Under these conditions,17 kinds of amino acids were separated completely in 55 minutes and 14 kinds of amino acids such as Asp,Glu,Ala,Leu and so on in the hydrolysate from fish protein hydrolysis in subcritical water were determined.
- subcritical water,
- biomass,
- solid waste,
- HPLC,
- amino acid,
- analytical chemistry
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